🧬 Chapter 8: Microbes in Human Welfare– Class 12--4 Marks Questions with Answers |NCERT + NEET Focus

Rashmi Mishra
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🌸Chapter 8

Microbes in Human Welfare

(4 Marks) 

1. Explain the role of microbes in the household products.

Answer:
Microbes are used in several household products:

1.   CurdLactobacillus converts lactose into lactic acid, coagulating milk.

2.   BreadSaccharomyces cerevisiae (yeast) ferments dough → CO₂ makes bread fluffy.

3.   BeveragesSaccharomyces cerevisiae ferments sugars to produce alcohol in beer, wine.

4.   CheeseLactobacillus and Penicillium roqueforti help in flavor and texture formation.


2. Describe the role of microbes in industrial production.

Answer:
Microbes produce various valuable products:

  • Alcoholic beveragesSaccharomyces cerevisiae.
  • Organic acidsAspergillus niger → citric acid; Acetobacter aceti → acetic acid.
  • EnzymesAspergillus → amylase; Bacillus → protease.
  • AntibioticsPenicillium notatum → penicillin.
    These are produced in large bioreactors under controlled conditions.

3. What is fermentation? Explain with industrial examples.

Answer:
Fermentation is the anaerobic conversion of sugar into alcohol or acids by microbes.
Examples:

  • Saccharomyces cerevisiae → sugar → ethanol (in brewing industry).
  • Acetobacter aceti → ethanol → acetic acid (vinegar).
  • Aspergillus niger → sugar → citric acid.
    Fermentation is used in making wine, beer, and vinegar.

4. Explain the discovery and importance of Penicillin.

Answer:

  • Discovered by Alexander Fleming in 1928 from Penicillium notatum.
  • Chain and Florey developed it for mass production.
  • It was the first true antibiotic, saved millions of lives during World War II.
  • Effective against Gram-positive bacteria by inhibiting cell wall synthesis.

5. Describe the steps of sewage treatment.

Answer:

1.   Primary treatment:

o   Physical removal of large particles by filtration and sedimentation.

2.   Secondary treatment:

o   Biological process using aerobic microbes.

o   Aeration tank → microbes oxidize organic matter → activated sludge.

3.   Sludge digestion:

o   Anaerobic microbes digest sludge → produce biogas.

4.   Effluent discharge:

o   Treated water released into rivers or reused.


6. Explain the role of microbes in sewage treatment plants.

Answer:

  • Aerobic bacteria oxidize organic matter → CO₂, H₂O.
  • Forms activated sludge, which settles and is partly reused.
  • Anaerobic microbes in digesters break down sludge → biogas (methane, CO₂).
  • Reduces organic load and prevents water pollution.

7. What is biogas? Describe its composition and production process.

Answer:
Biogas is a methane-rich fuel produced by anaerobic digestion of organic waste.

  • Composition: 50–70% CH₄, 30–40% CO₂, and traces of H₂S.
    Process:

1.   Slurry of cow dung + water fed into digester.

2.   Anaerobic microbes (methanogens like Methanobacterium) decompose it.

3.   Biogas collected from the dome and used as fuel.


8. Explain the working of a biogas plant.

Answer:

1.   Mixing tank – Cow dung + water slurry prepared.

2.   Digester tank – Anaerobic bacteria break down organic matter.

3.   Gas holder – Biogas accumulates in dome.

4.   Outlet – Used slurry removed and used as manure.
Biogas contains methane and is used for cooking and lighting.


9. What are methanogens? Where are they found and what are their uses?

Answer:

  • Methanogens are anaerobic archaebacteria producing methane gas.
  • Example: Methanobacterium.
  • Found in:
    • Rumen of cattle → digest cellulose.
    • Anaerobic digesters in sewage plants → produce biogas.
  • Used for waste management and renewable energy.

10. Explain how microbes are useful in biogas production and biocontrol.

Answer:

  • Biogas: Methanobacterium decomposes organic waste → methane.
  • Biocontrol: Trichoderma fungi kill soil pathogens; Bacillus thuringiensis kills pests.
    Both methods are eco-friendly and reduce chemical usage.

11. Describe the different types of biofertilizers and their roles.

Answer:

1.   Symbiotic nitrogen fixersRhizobium in legume root nodules.

2.   Free-living nitrogen fixersAzospirillum, Azotobacter.

3.   CyanobacteriaAnabaena, Nostoc, Oscillatoria (in paddy fields).

4.   Mycorrhizae – Fungal associations aiding phosphorus absorption.


12. Differentiate between chemical fertilizers and biofertilizers.

Chemical Fertilizers

Biofertilizers

Inorganic salts providing NPK.

Living microbes improving fertility.

Quick effect but temporary.

Slow but sustainable.

Cause soil and water pollution.

Eco-friendly and renewable.


13. Explain the role of microbes as biofertilizers in agriculture.

Answer:

  • Rhizobium fixes nitrogen in legumes.
  • Azotobacter and Azospirillum fix nitrogen freely in soil.
  • Anabaena in Azolla enriches paddy fields.
  • Mycorrhiza increases phosphate absorption.
    → Improves soil fertility and reduces chemical use.

14. What is bio-control? Describe with examples.

Answer:
Use of living organisms to control pests and plant diseases.
Examples:

  • Bacillus thuringiensis → kills insect larvae (Bt cotton).
  • Trichoderma → controls soil pathogens.
  • Ladybird beetles → feed on aphids.
    Eco-friendly alternative to pesticides.

15. Explain how Bacillus thuringiensis helps in pest control.

Answer:

  • Produces Cry toxin protein crystals.
  • When ingested by insects, toxin binds gut lining → forms pores → cell death.
  • Used in sprays and Bt cotton for caterpillar control.

16. How are microbes used in organic farming?

Answer:

  • Uses Rhizobium, Azospirillum, Anabaena as biofertilizers.
  • Trichoderma, Bt as biocontrol agents.
  • Avoids chemical fertilizers/pesticides → sustainable farming.
  • Improves soil structure and fertility.

17. Describe the role of microbes in industrial enzyme production.

Answer:

  • Microbes secrete enzymes used in industries:
    • Amylase → starch hydrolysis.
    • Lipase → detergents and food processing.
    • Pectinase → fruit juice clarification.
    • Protease → leather and textile industries.

18. What is a bioreactor? Describe its function.

Answer:

  • Large vessel for large-scale microbial product synthesis.
  • Provides optimal conditions: temperature, pH, aeration, agitation.
  • Has inlet for nutrients, air supply, pH sensors, and outlet for product.
  • Used in production of antibiotics, enzymes, alcohol.

19. What is an antibiotic? Name some important examples and their uses.

Answer:

  • Chemical substances that destroy/inhibit other microbes.
    Examples:

1.   Penicillin – Penicillium notatum – antibacterial.

2.   Streptomycin – Streptomyces griseus – TB treatment.

3.   Tetracycline – Streptomyces aureofaciens – broad-spectrum.


20. Write a short note on Rhizobium and Anabaena.

Answer:

  • Rhizobium: Symbiotic nitrogen fixer in legumes; forms nodules.
  • Anabaena: Cyanobacterium; fixes nitrogen independently or symbiotically with Azolla.
    Both enrich soil with nitrogen, improving fertility naturally.

21. What is the significance of microbes in waste management?

Answer:

  • Microbes decompose organic waste in compost pits.
  • Sewage treatment plants use bacteria to remove organic matter.
  • Methanogens produce biogas from waste.
  • Reduce pollution and recycle nutrients.

22. Describe the process of secondary sewage treatment.

Answer:

1.   Sewage passed into aeration tank.

2.   Air pumped → promotes growth of aerobic microbes.

3.   Organic matter oxidized → CO₂ + H₂O.

4.   Activated sludge formed → settled → some reused; rest digested anaerobically.


23. Explain the role of microbes in the dairy industry.

Answer:

  • Lactobacillus and Streptococcus convert milk → curd, yogurt.
  • Propionibacterium sharmanii → Swiss cheese (holes).
  • Penicillium roqueforti → blue cheese flavor.
  • Improve texture, flavor, and digestibility.

24. Name the microbes involved in the production of:

(a) Wine
(b) Cheese
(c) Citric acid
(d) Curd
Answer:
(a) Saccharomyces cerevisiae
(b) Lactobacillus, Penicillium roqueforti
(c) Aspergillus niger
(d) Lactobacillus bulgaricus


25. How is organic waste converted into energy by microbes?

Answer:

  • Anaerobic bacteria digest waste in biogas plants → produce methane (biogas).
  • Methanogens like Methanobacterium act on cow dung, sewage sludge.
  • Biogas used as renewable fuel; residue → manure.

26. What is the function of the activated sludge in sewage treatment?

Answer:

  • Contains aerobic microbes that oxidize organic matter.
  • Used as inoculum for further treatment.
  • Remaining sludge digested anaerobically to generate biogas.

27. How do microbes help in retting of jute and flax?

Answer:

  • Clostridium species decompose pectins binding fibers to stem.
  • Loosens fibers for easy extraction.
  • Essential for jute/flax fiber processing.

28. Discuss the uses of microbes in the pharmaceutical industry.

Answer:

  • Antibiotics: Penicillin, Streptomycin.
  • Vaccines: Produced using attenuated microbes.
  • Enzymes: Proteases and lipases for drug synthesis.
  • Vitamins: B₁₂, riboflavin produced by bacteria.

29. Why are antibiotics ineffective against viruses?

Answer:

  • Viruses lack cell walls and metabolic machinery.
  • Antibiotics target bacterial processes like protein/cell wall synthesis.
  • Hence, they cannot act on viruses.

30. What are single-cell proteins (SCP)? How are they beneficial?

Answer:

  • Microbial biomass rich in protein used as food.
  • Example: Spirulina, Methylophilus methylotrophus.
    Benefits:
  • High protein yield.
  • Rich in vitamins/minerals.
  • Useful for malnutrition control.

31. Explain the importance of cyanobacteria in agriculture.

Answer:

  • Anabaena, Nostoc, Oscillatoria fix atmospheric nitrogen.
  • Increase soil fertility in paddy fields.
  • Used as biofertilizers and reduce chemical fertilizer need.

32. What is the importance of Azolla-Anabaena association in rice fields?

Answer:

  • Azolla (water fern) hosts Anabaena (cyanobacteria).
  • Anabaena fixes nitrogen, enriching soil naturally.
  • Used as green manure in paddy cultivation.

33. What are probiotics? Mention their benefits.

Answer:

  • Live beneficial microbes improving gut health.
  • Example: Lactobacillus acidophilus.
    Benefits:
  • Boost immunity.
  • Prevent diarrhea, improve digestion.
  • Suppress harmful gut microbes.

34. How does Trichoderma act as a bio-control agent?

Answer:

  • Fungus living in plant root ecosystems.
  • Produces antifungal metabolites that suppress pathogens.
  • Used in seed and soil treatments for crop protection.

35. Describe the role of microbes in food and beverage industries.

Answer:

  • Yeast → beer, wine.
  • Lactobacillus → yogurt, curd.
  • Aspergillus oryzae → soy sauce.
  • Acetobacter → vinegar.
    Enhances taste, flavor, and preservation.

36. Explain how antibiotics are produced on a large scale.

Answer:

  • Grown in large fermenters with nutrient medium.
  • Microbes produce antibiotic during stationary phase.
  • Extracted, purified, and processed into medicines.

37. How do microbes contribute to soil fertility?

Answer:

  • Fix nitrogen (e.g., Rhizobium, Azotobacter).
  • Decompose organic matter → humus formation.
  • Release nutrients (phosphorus, sulfur) for plant uptake.

38. What is the use of Aspergillus niger in industries?

Answer:

  • Produces citric acid (food preservative).
  • Secretes enzymes like amylase and pectinase.
  • Used in beverage and detergent industries.

39. How do microbes help in bioremediation?

Answer:

  • Microbes degrade toxic pollutants.
  • Pseudomonas breaks down petroleum.
  • Used in oil spill cleanup and waste treatment.

40. Write short notes on microbial enzymes used in industry.

Answer:

  • Amylases: break starch (food/textile).
  • Lipases: used in detergents.
  • Pectinases: juice clarification.
  • Proteases: used in leather/textiles.

41. How are microbes useful in the textile industry?

Answer:

  • Amylase → starch removal from fabric.
  • Protease → softens silk and wool.
  • Pectinase → retting of fibers.

42. Describe the role of microbes in alcohol production.

Answer:

  • Yeast (Saccharomyces cerevisiae) ferments sugar → ethanol + CO₂.
  • Used in wine, beer, whiskey industries.
  • Anaerobic process in fermentation tanks.

43. What are the advantages of using biofertilizers in agriculture?

Answer:

  • Eco-friendly and non-polluting.
  • Improve soil structure and fertility.
  • Cost-effective and sustainable.
  • Promote long-term yield stability.

44. Describe the working of a typical biogas plant with labeled parts.

Answer:

1.   Mixing tank (cow dung + water).

2.   Digester (anaerobic microbes act).

3.   Gas holder (stores methane).

4.   Outlet (for digested slurry).
Methane used as fuel; slurry as manure.


45. Explain how microbes are used in the production of citric acid and vinegar.

Answer:

  • Citric acid: Aspergillus niger ferments sugar.
  • Vinegar: Acetobacter aceti oxidizes ethanol → acetic acid.
    Used in food and beverage industries.

46. Describe the importance of Trichoderma and Bacillus thuringiensis in agriculture.

Answer:

  • Trichoderma – controls soil fungi (biofungicide).
  • Bt – insecticidal protein kills caterpillars.
    Both are eco-friendly alternatives to chemicals.

47. Write the principle behind biogas production.

Answer:

  • Anaerobic digestion of organic waste by methanogens.
  • Converts biomass into methane-rich gas.
  • Methane used as renewable energy source.

48. How do microbes help in improving the quality of curd and cheese?

Answer:

  • Lactobacillus ferments lactose → lactic acid → curd formation.
  • Propionibacterium and Penicillium enhance cheese flavor and texture.

49. What are the uses of fungi in industry?

Answer:

  • Penicillium → antibiotics.
  • Aspergillus niger → citric acid.
  • Trichoderma → biofungicide.
  • Saccharomyces cerevisiae → alcohol production.

50. Describe the process and advantages of biogas production.

Answer:

  • Cow dung + water → anaerobic digestion by Methanobacterium.
  • Produces methane-rich gas for cooking and lighting.
    Advantages:

1.   Renewable energy source.

2.   Reduces waste.

3.   Provides organic manure.


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